Ogg1 activity kit

Ogg1 activity kit. 9 vs. 2. We did not detect any glycosylase activity of β-Ogg1 in this study under any of the numerous conditions tested (see below). Oct 19, 2004 · However, those experiments employed very high concentrations of recombinant β-Ogg1 (1 μg, 20 pmol) compared to substrate (75 fmol), resulting in a specific activity of β-Ogg1 that was 100-fold lower than that of Fpg. Aug 21, 2006 · Chronic exposure to low doses of cadmium lowers the cellular activity of OGG1 in animal models (36, 37) or in human cells . The provided reagents and supplies allow personnel to Enzymatic activity excising 8-oxoguanine from DNA (OGG activity) was reduced in peripheral blood mononuclear cells (PBMCs), and in paired lung tissue, from patients with non–small cell lung cancer. Oxidative DNA damage is primarily repaired via the base excision repair pathway that is initiated by DNA glycosylases. DNJ inhibits α-glucosidase, reduces the absorption of sugar, and suppresses after-meal hyperglycemia. Interestingly, under the conditions of our assay, preincubation of OGG1 with PAR did not significantly affect OGG1 incision activity (Fig. Sep 1, 2016 · OGG1 is essential for H 2 O 2 induced DNA demethylation in MEF cells. 1). This review will concentrate on adverse deleterious health effects that are associated with both the diminution of OGG1 activity via population-specific polymorphic variants and the complete loss of OGG1 in murine models. Patients with the CC genotype exhibited shorter relapse-free survival (RFS). Among these, 8-oxoguanine glycosylase (OGG1), the major DNA glycosylase, is responsible for removal of 7,8-dihydro-8-oxo-2’-deoxyguanosine (8-OH-dG), one of the most frequent endogenous base lesions formed in the DNA of aerobic organisms. 0165 or Fax +1. In the former cases, the cadmium-induced reduction of OGG1 activity was paralleled by lower enzyme levels most likely resulting from inhibition of gene expression at the transcriptional level. (a) Deacetylation of OGG1 by Sirt3 in vivo: The human glioma cells LN229 were transfected with a Flag-Sirt3 expression plasmid or a May 31, 2024 · Of note, OGG1 can increase α-SMA level through promoting α-SMA polymerization into stress fibers (Luo et al. This mouse model displays diet-and age-related induction of metabolic syndrome, highlighting a key role for OGG1 in Jun 23, 2022 · To test if TH10785 stimulates the OGG1 β-lyase activity on AP sites, we performed the assay using a uracil-containing substrate in the presence of human uracil-DNA glycosylase 2 (UNG2). 5 A), suggesting that PARP-1 is important for modulating OGG1 activity. c Schematic of the repair enzyme-based assay for 8-oxoG quantification in DNA. The mechanism highlighted herein may be explained by some of our previous work demonstrating that OGG1 promotes NF-κB activity by increasing DNA occupancy on the κB site . 0166 May 24, 2018 · Previous studies from our and others' groups presented that OGG1-BER activity is redox regulated 48,49,50. It was reported that DNJ functions in attenuating cellular oxidative stress. We found that 10 of them failed to enhance or partially lost their stimulatory effects on OGG1 promoter activity (Figure 6D). Precautions for Kits 1. Induction of OGG1 activity is not a result of induction of OGG1 gene expression as assessed by qPCR suggesting a role for protein stabilisation or increased OGG1 catalytic activity. Jul 24, 2018 · The enzymatic activity of the lysine-specific demethylase LSD1 gives rise to a localized generation of H 2 O 2, which oxidize G to 8-oxoG. The assay is based on Ogg1 glycosylase activity that recognizes and removes the mutated base (8-oxo-G). In comparison to sham treatment, siRNA transfection was associated with a substantial reduction in Ogg1 mRNA that persisted for at least 48h. OGG1's promoter enrichment, oxidative state, and gene expression were examined. Aug 8, 2023 · Another report demonstrates that C28 oxidation-induced OGG1 dimerization, which does not impede substrate recognition but suppresses OGG1 catalytic activity at 8-oxoGua, results in enhancement of Myc recruitment to its E-box promoter recognition sequences . Together, these studies Jan 1, 2016 · We have used the 1-α isoform (transcript variant 1a) of OGG1 as it displays DNA glycosylase activity in both the nucleus and mitochondria, as opposed to the mitochondria localized β-OGG1(transcript variant 2a), which has been reported to lack such activity . OGG1 is also demonstrated to promote lung fibrosis via regulating TGFβ/SMAD signaling and activating fibroblasts (Wang et al. 2023). 8-oxoG was measured by ELISA GA103313G2, OGG1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa. The kit is designed based on the best knowledge of Human Ogg1 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the <i>in vitro</i> quantitative determination of human Ogg1 in serum, plasma, tissue homogenates, and other biological fluids. Genomic Aug 29, 2022 · Allosteric activation of OGG1 AP-lyase activity mediated by the product of the OGG1 glycosylase reaction and its analogs, as shown herein, may provide a positive feedback regulation mechanism to Apr 1, 2018 · Moreover, OGG1 activity is also regulated by posttranslational modifications (Fig. Radicella) was used to determine OGG1 gene transcription activity. However, the mechanisms remain largely unknown. The relative OGG1 promoter activity in cells overexpressing the 10 mutants was at most 70% of that in cells overexpressing SIRT2 WT. Thus, our results which confirmed that targeting of hOGG, isoform 1-α into Feb 1, 2024 · As both marginal and severe Zn deficiency decreased OGG1 protein, we further investigated if this influences the catalytic activity of OGG1. The substrate is a 23 oligonucleotide containing 8-oxo-dG at its 11th base, labeled with 32 P at its 5′ end, and annealed to its complementary strand (containing dC at the opposite base position to the 8-oxo-dG). OGG1 gene is associated with the risk of various cancers such as lung and prostate cancer. cultured cells using the Flexigene DNA Kit Human 8-oxoGuanine DNA Glycosylase / OGG1 ELISA Kit. detected a Ser326 /Cys326 polymorphism the OGG1 polymorphism found in the same cell pop- associated with decreased OGG1 activity upon ex- ulation, we observed that there was no association pression of OGG1 in E. OGG1 Aug 7, 2015 · There is a growing body of evidence that suggests inhibition of 8-oxoguanine DNA glycosylase-1 (OGG1) could be useful as a mono-therapy or in combination therapy to treat certain types of cancer. TBB5 was used as an internal control. OGG1 prevents the accumulation of mutations and regulates the transcription of various oxidative stress–response genes. 1 right). 2020; Song et al. OGG1; 8-oxoguanine DNA glycosylase: Aliases: HMMH, MUTM, OGH1, HOGG1 : Location: 3p25. Subsequently, OGG1-initiated base excision creates strand break, which serves as an entry for Top IIβ, triggering DNA conformational changes to accommodate the transcription initiation complex. 35–12. Jan 3, 2016 · Representative images of each genotype of PyMT mice with mammary tumors obtained from 12-16-week old female (S1); Histological sections of the lungs from PyMT mice of different OGG1 genotypes (S2); OGG1 activity in mammary tumors isolated from B-nuclear, and D- mitochondrial fractions from PyMT/KO and PyMT/Tg animals (S3); LDH activity in Feb 2, 2023 · OGG1 loss-of-function does not affect TFs expression and nuclear translocation, as shown with SMAD3 and NF-κB (Supplementary Figures S9 and S10). The action of this enzyme includes lyase activity for chain cleavage. Apr 27, 2017 · After exposure to IR, NO can inhibit Ogg1 activity via nitrosylation of the zinc-finger motif of this enzyme [127, 128]. Jun 7, 2005 · To analyze the effect of OGG1 phosphorylation by c-Abl on its catalytic activity, we assayed 8-oxoG incision of OGG1 incubated with active (Figure 7B, lanes 3–8) or heat-inactivated (lanes 9–14) c-Abl. As expected, no incision activity against 8-OxoG was detected in mitochondrial or nuclear extracts from KO mice (Figure 1 Sep 9, 2016 · A luciferase reporter plasmid containing the OGG1 promoter (courtesy of Dr. Our previous work has established that OGG1 can augment a subset of cytokine and chemokine gene expression Apr 10, 2024 · 1-Deoxynojirimycin (DNJ) is a type of alkaloid that mainly exists in mulberry fruit and leaves. Enzyme-linked immunosorbent assays identify and calculate substances such as proteins, peptides, antibodies, and hormones. L-arginine treatment promoted and restored the production of OGG1 at Day 8 and Day 12 and L-NAME inhibited the production of OGG1 from Day 4 after ozone stress (Figure 4C). The different steps involved in human OGG1 is regulated by cadmium through suppression of Sp1 activity; OGG1 and Nei-like endonuclease (NEI1), DNA glycosylases which do not stably interact, stimulate 8-oxoguanine repair by a collaboration that is possible because of higher abasic (AP) site affinity and stronger AP lyase activity of NEI1 relative to OGG1. 01]. In addition to targeting DNA, oxidative stress can affect proteins like OGG1 itself, specifically at OGG1 gene is associated with the risk of various cancers such as lung and prostate cancer. 8-oxoGuanineDNAGlycosylase/OGG1 removes 8-oxoguanine, a mutagenic base byproduct that occurs when cells are exposed to reactive oxygen. In a cross-sectional study Dec 30, 2022 · Inhibition of OGG1 with TH5487 interferes with OGG1 incision activity, resulting in fewer DNA double-strand breaks in cells exposed to oxidative stress and reducing the DNA mutation rate caused by OGG1 exposure to AP sites, which may reduce the incidence of cancer in cases of oxidative stress damage . As such, excessive OGG1 activity could theoretically overcome the repair capacity of APE1 which could lead to excessive numbers of AP sites, triggering single- and double-strand breaks and large mtDNA deletions. [25] Nov 22, 2023 · It is unknown how the DNA repair enzyme OGG1 relates to healthy aging in humans, in particular to inflammaging, that is associated with increased levels of TNF-α. Mar 7, 2017 · OGG1 plays a key role in providing the transcriptional activity from the Cxcl2 promoter. We acetylated recombinant OGG1 in vitro by incubation Mar 3, 2021 · Given the significant literature that associates deficiencies in OGG1-initiated repair with positive prognostic indicators in AML and other diseases, this investigation was designed to establish a mechanistic link between the levels of OGG1 activity and chemotherapeutic responsiveness of specific AML subtypes and thus validate OGG1 as a novel . GA103313G3, OGG1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa. 9%, odds ratio (OR) = 4. OGG1 has a beta-lyase activity that nicks DNA 3' to the lesion. 8. Here, the authors describe the use of a small molecule OGG1 inhibitor Feb 1, 2023 · The different models involve or not the excision of 8-oxoG by OGG1, the dynamic folding of G-quadruplex structures as a consequence of OGG1 activity, or the SSB generated by the enzymatic activity of both OGG1 and APE1, although many more studies are needed to fully understand the mechanistic details (Fig. OGG1 is acetylated in vivo by histone acetyltransferases, such as cyclic AMP response element-binding protein/p300 complex (CBP/p300), and the acetylation at Lys338/Lys341 significantly increases OGG1 activity. Aug 6, 2024 · Complete information for OGG1 gene (Protein Coding), 8-Oxoguanine DNA Glycosylase, including: function, proteins, disorders, pathways, orthologs, and expression. Ogg1 mRNA abundance and Ogg1-like activity in sham and siRNA-transfected PAECs are shown in FIGURE 1A and B, respectively. The YF594 TUNEL Assay Apoptosis Detection Kit (US Everbright Inc. S4), suggesting a stimulation of the residual β-lyase activity of OGG1. 70, P = 0. Feb 10, 2021 · The recently developed OGG1 inhibitor TH5487 has proved to bind efficiently to the catalytic site of OGG1 blocking its enzymatic activity 23,24. Jan 1, 2016 · Production of mitochondrial reactive oxygen species and integrity of mitochondrial DNA (mtDNA) are crucial in breast cancer progression and metastasis. (Fig. 8-Oxoguanine glycosylase (OGG1) is a base excision repair enzyme responsible for the recognition and removal of 8-oxoguanine, a commonly occurring oxidized DNA modification. 1b); 1b); it excises 8-oxo-dG opposite to the cytosine base and generates an AP site 10. After opening the kit, please refer to the table of storage condition for coated plate and standards (Dampness may decrease the activity. Although the expression of OGG1 was significantly increased in the lung tissue on the 21st and 28th day after BLM induced lung fibrosis in mice [12], the changes in OGG1 expression during BLM-induced fibrosis within 21 days were not explored. This study aimed (1) to investigate how 24-h profiles for OGG1 change during healthy aging and (2) to analyze the relationship of OGG1 with TNF-α, central body fat, cortisol and oxidative DNA/RNA damage. ) was used to label Oct 5, 2018 · OGG1 activity has also been shown to be important to DNA total DNA was isolated using the Qiagen DNA isolation kit from eWAT pooled from 6–8 animals per cohort. OGG1 is a bifunctional DNA glycosylase capable of cleaving the 3′-end of the AP site; 3′-deoxyribose phosphate (3′-dRP) and 5′-phosphate are produced via a β-elimination mechanism. The AP sites generated by UNG2 were resolved with increasing efficiency as well (fig. High resolution confocal microscopy pinpointed the location of the fluorescent molecular beacon in live cells to perinuclear regions that were identified as May 8, 2018 · The OGG1 S326C CC genotype (associated with lower OGG1 activity) was observed more frequently in patients with AML relapse [28. We explored whether 8-oxoG accumulation in aged Hdac1 cKO mice could be due to altered OGG1 activity. In addition, the Ogg1 control enzyme provided with the kit, enables screening of Ogg1 modulators. Next, we examined the effect of miR-33 inhibition on OGG1 activity to repair oxidized DNA damage. The Ogg1 activity assay is based on General description. When using different Elisa kits, labeling is required to avoid mixed components and failed assay. 8-oxoguanine DNA glycosylase (OGG1) recognizes and cleaves oxidized and ring-fragmented purines, including 8-oxoguanine, the most Jan 18, 2023 · OGG1 loss-of-function does not affect TFs expression and nuclear translocation, as shown with SMAD3 and NF-κB (Supplementary Figures S9 and S10). 858. 4B). The Ogg1 activity assay is based on The ELISA analytical biochemical technique of the MBS702793 kit is based on OGG1 antibody-OGG1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OGG1 antigen targets in samples. These data imply that under oxidizing or inflammatory conditions, OGG1 is responsible May 18, 2020 · The washed beads with bound HDAC1 or p300 were assessed for HDAC or histone acetylase activity using the FLUOR DE LYS® HDAC Fluorometric Activity Assay Kit (Enzo Life Sciences) or HAT Activity Jul 20, 2017 · Oxidative stress resulting from endogenous and exogenous sources causes damage to cellular components, including genomic and mitochondrial DNA. P. The complexity function of OGG1 may lead to different roles in lung fibrosis. 127 Personalized treatment approaches are also paramount given the complexity and diversity of clinical presentations. Dec 12, 2011 · In addition, similar to these results, PARP-1 impaired OGG1 activity when incubated with NAD + alone (data not shown). Therefore, we evaluated the role of mtDNA damage in breast cancer by genetically modulating the DNA repair enzyme 8-oxoguanine DNA glycosylase (OGG1 … May 6, 2020 · The present study aimed to examine if BER activity of OGG1 is required for pro‐inflammatory gene expression. By using immunofluorescence, we found OGG1 Aug 9, 2024 · A decrease in OGG1 activity is observed in early AD, suggesting that early intervention to boost OGG1 activity could be critical for preventing AD progression. The OGG1 level were lower in ozone group compared to control group at Day 0; But, L-arginine promoted and restored the levels of OGG1 from Day 4 and L-NAME inhibited the production of OGG1 at Day 8 and Day 12 after ozone stress (Figure (Figure4B). Moreover, lung tissues were Aug 10, 2023 · To evaluate the influence of other blood cells and coagulation activity on the by a commercial ELISA kit (Mouse von Willebrand Factor ELISA Kit, CSB-E08439m, CUSABIO) according to the OGG1; OGG1 Type 1d; OGG1 Type 1e; OGG1 Type 1f; OGG1 Type 1g; OGG1 Type 1h; OGH1; Description 8-Oxoguanine DNA Glycosylase, or OGG1, is a DNA repair enzyme that removes both 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. May 18, 2020 · OGG1 can be acetylated by the histone acetyltransferase (HAT) p300, which stimulates OGG1 activity, as well as deacetylated by HDAC1, presumably on lysine residues acetylated by p300 47. 4 The Ogg1 Assay Kit is designed for Ogg1 activity detection in cells lysates or for assaying purified enzyme preparations. Tyrosine phosphorylation of OGG1 under these conditions was again confirmed by western blot (Figure 7A, lanes 2–4). [24] OGG activity was also reduced in PBMCs of patients with head and neck squamous cell carcinoma (HNSCC). 3: Summary: This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. To investigate the role of 8-oxoG accumulated May 1, 2011 · Ogg1 mRNA expression and Ogg1-like activity in siRNA transfected PAECs. Therefore, OGG1 has been considered as a critical mediator in the Sep 2, 2016 · Cotransfection of miR-33-3p and -5p mimics markedly suppressed the activity of the OGG1 3′UTR luciferase construct, compared with empty vector control (Figure 7E). Together, these studies Apr 26, 2017 · A 38 kDa protein corresponding to OGG1 were detected and decreased OGG1 were detected in ozone-induced lung injury animal models. The ELISA Kit is designed to detect native, not recombinant, OGG1. In this study, we firstly confirmed that 5 µmol/L DNJ treatment Feb 21, 2022 · a, b Immunofluorescence and quantification of 8-oxoG in cells transfected with control, DDB2 or OGG1 siRNA. 13 OGG1 deficiency leads to elevated levels of 8-OH-dG Jan 29, 2024 · Studies have confirmed that HDAC1 interacts with and deacetylates 8-oxoGDNA glycosylase 1 (OGG1), the key DNA oxidative damage repair enzyme, enhancing its cleavage activity [47, 48]. 10, 95% confidence interval (CI) = 1. ). OGG1 localizes to both nucleus and mitochondria [8], [40], therefore, subcellular fractions were prepared, and fraction purity determined by western blotting against specific subcellular markers indicated that pure nuclear and mitochondrial fractions were Mar 23, 2021 · Mth1/Ogg1 knockout in 3xTg-AD-H mice induces neurodegeneration accompanied with Aβ accumulation in the brain, and exacerbates cognitive impairment. 633. Jul 18, 2013 · Effects of Sirt3 on deacetylation and incision activity of OGG1. Buy OGG1 assay kit, Mouse Oxoguanine Glycosylase 1 (MBS2158807) product datasheet at MyBioSource, Assay Kits Call +1. Oct 21, 2022 · OGG1 is primarily responsible for removing 8-oxo-dG (Fig. 1 CRISPRa-Enhancer vector, SKU GE100056. 29 Human Embryonic Kidney 293 (HEK 293) cells were grown in 12-well plates to 60–70% confluence, then co-transfected with both renilla and OGG1 plasmid covering the region of Nrf2, −35–21, that Feb 1, 2014 · OGG1 carries out the recognition and removal of 8-OHdG, leading to the formation of AP sites, which are then processed by APE1. In contrast to our Feb 6, 2023 · Idiopathic pulmonary fibrosis is a disease caused by persistent micro-injuries to the lung ultimately resulting in death. (B) MEF WT or Ogg1 −/− cells were treated with H 2 O 2 or PBS for 6 h. (A) Verification of OGG1 levels in MEF WT and Ogg1 −/− cells was measured using anti-OGG1 antibody. If any component is missing or damaged during the assay or storage, Jun 7, 2013 · On the other hand, no difference in OGG1 activity was observed in lanes containing nuclear fractions, thus confirming that hOGG1 is predominantly expressed in mitochondria without leakage expression in nucleus (Figure 1 B). 2014). It is necessary to carefully consider whether an OGG1-based Jul 6, 2016 · Oxidative damage in DNA is repaired primarily via the enzymes of the base excision repair pathway. Apr 27, 2017 · The activities of the OGG1 were determined by using ELISA. To this end, Ogg1/OGG1 knockout/depleted cells were transfected with constructs expressing wild‐type (wt) and repair‐deficient mutants of OGG1. Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. coli defective in the repair of between OGG1 activity and Ser326 /Cys326 Mar 30, 2024 · We then further examined whether these SIRT2 mutants affected OGG1 promoter activity. Jan 5, 2024 · The function of OGG1 far exceeds its DNA repair [9]. 1 CRISPRa scramble vector, SKU GE100077. Due to the involvement of ogg1 in base excision repair (BER) pathway via Comparing the OGG1 activity in lymphocytes with Kohno et al. 8-oxoGuanineDNAGlycosylase/OGG1 contains a mitochondrial targeting signal for mitochondrial localization and lyase activity to cleave chains. To identify inhibitors of OGG1, a fluorescence-based screen was developed to analyze OGG1 activity in a high-throughput manner. ass ymefvh amer dixs marhk ehesshzck qbemf uries rul mmu