Dna nanostructures review. The prevalent B-form structure of DNA manifests as a double-stranded helix measuring ≈2 nm (diameter), with 3. 3 days ago · This review provides an overview of the various applications of DNA nanostructures, including scaffolded DNA, DNA hydrogels, tetrahedral DNA nanostructures, DNA origami, spherical nucleic acids, and other DNA-based nanostructures in cancer immunotherapy. Aug 29, 2023 · Liposomes are indispensable model membranes and drug carriers. Theory suggests that the circular dichroism spectra Jul 7, 2024 · DNA origami nanostructures (DOs) are promising tools for applications including drug delivery, biosensing, detecting biomolecules, and probing chromatin substructures. • Synthetic membrane channels made of DNA are reviewed. Theory suggests that the circular dichroism spectra . In this review, we highlight the current progress in the design and application of three DNA-based nanostructures: DNA origami, lipid-nanoparticle (LNP) technology related to frame guided assembly (FGA), and DNA hydrogel for the delivery of RNA molecules. This Review addresses the use of DNA triplexes to assemble sensing platforms and molecular switches. Fan et al. However, the correlation between DNA-origami design and internalization efficiency in different cancer cell lines remains Oct 1, 2015 · The field of structural DNA nanotechnology started around 30 years ago when Ned Seeman performed pioneering research with DNA junctions and lattices [1]. Controlled mechanochemical coupling of anti Jun 17, 2024 · Here we review the existing literature on promoter-independent transcription by bacteriophage RNA polymerases with a specific focus on T7 RNAP, and provide examples of how promoter-independent reactions can disrupt the functionality of DNA strand displacement circuit components and alter the stability and functionality of DNA-based materials. Self-Assembly of DNA Nanoparticle Nanostructures through Hybridization or DNA Protein Interactions P 6. In this perspective, DNA nanostructure-based probes are reviewed and summarized from several aspects: probe classification according to the dimensions of DNA nanostructures (one, two, and three-dimensional nanostructures), the common connection modes between nucleic acid probes and DNA nanostructures, and the most important advantages of DNA Aug 1, 2024 · This review explores the advancements and applications of DNA nanostructures in cancer treatment, with a focus on targeted drug delivery and multi-drug therapy. DNA nanostructures can Dec 10, 2021 · This review is based on the development of DNA nanodevices and, as shown in Figure 1 in recent years, lists several kinds of DNA nanostructures used for drug carriers, and summarizes the applications of DNA nanodevices responding to physical, chemical, and biological stimuli in the fields of tumor treatment and biological imaging, and looks Mar 1, 2024 · Of various DNA nanostructures, DNA tetrahedron has become one of the commonly-used nanomaterials in the bioanalysis field due to its advantages of efficient uptake by cells, high solubility, biocompatibility and responsiveness [101, 102]. Conclusions and Perspectives Y Author Jan 18, 2021 · In this review, first the state of the art of functional DNA nanostructures is summarized, and then the overview of the use of DNA nanostructures to engineer the cell membrane is presented. We then propose an outlook to drive the translation of DNA constructs toward preclinical design. Dec 19, 2022 · The DNA origami method enables high-yield fabrication of DNA nanostructures at the 1–100-nm length scale 1,2. Because of their programmable assembly and precise control of size, shape, and function, DNA particles can be used for numerous biological applications, including imaging, sensing, and drug delivery. Latest Research and Reviews. Jan 10, 2012 · Recent Advances in DNA Nanostructures Applied in Sensing Interfaces and Cellular Imaging. This Review describes how DNA nanostructures template calcium-based and silica biominerals, with applications envisioned in biomedicine Jan 28, 2021 · F,G | Examples of dynamic DNA origami nanostructures: a DNA origami box 89 whose lid is initially locked by two DNA duplexes and can be opened via strand displacement by oligonucleotide keys (blue Aug 1, 2024 · This review explores the advancements and applications of DNA nanostructures in cancer treatment, with a focus on targeted drug delivery and multi-drug therapy. 4 base pairs) per helical turn. This review will provide a useful reference for the application of triangular unit-based DNA nanostructures in biosensing and provide ideas for designing and changing DNA nanostructures to improve the accuracy of biosensing. Designed DNA nanostructures In 1982, Seeman proposed to use DNA as the structural REVIEW ARTICLE Open Access Dynamic regulation of DNA nanostructures by noncanonical nucleic acids Shiliang He1, Zhilei Ge 2, Xiaolei Zuo 1,ChunhaiFan2 and Xiuhai Mao 1 Abstract May 7, 2024 · 1 DNA Nanostructures. Concurrently, an increased focus has emerged on the applications of DNA nanostructures. Sep 10, 2024 · DNA nanostructures are nanoscale structures made of DNA, which acts both as a structural and functional element. This enables drugs to access cancer cells with greater stability and precision, boosting treatment efficacy and lowering toxicity. Mar 29, 2022 · With excellent addressability and biocompatibility, DNA nanostructures are promising candidates for bio-sensing, bio-imaging, and drug delivery • The recent progress in chemical modifications of DNA nanostructures is summarized • Chemically modified DNA nanostructures for efficient delivery of chemotherapeutic drugs and nucleic acid drugs Aug 7, 2023 · Since DNA origami nanostructures (DONs) possess excellent editability, biocompatibility, and in vivo stability, they can be utilized to create DNA nanostructures with a variety of activities and anti-tumor medications. Jan 18, 2023 · The advances in DNA origami-based DNA nanotechnology were discussed in many review articles and monographs. Herein, this review is dedicated to offering a new perspective on using DNA nanostructures to explore the progress of cell–cell communication. While the biocompatibility, programmability, and ease of Nature uses extracellular matrix scaffolds to organize biominerals into hierarchical structures over various length scales. 3 days ago · Recently, DNA nanostructures have emerged as promising diagnostic and therapeutic platforms for cancer treatment. The potential and challenges associated with self-adjuvant DNA nanostructures will also be discussed. Bujold, 1,2 Aure´lie Lacroix, and Hanadi F. 25-bp DNA ladder; lanes 2, 4, 6, 8, and 10 are negative controls without the initiator, while lanes 1, 3, 5, 7, and 9 are Jun 6, 2022 · DNA nanotechnology has emerged as a powerful tool to precisely design and control molecular circuits, machines and nanostructures. It discusses the benefits and current limitations of nanoscale formulations in cancer therapy, categorizing DNA nanostructures into pure forms and hybrid versions optimized for drug Jan 13, 2021 · DNA is a promising scaffold for guiding biomineralization. In this method, a long single-stranded DNA (ssDNA) ‘scaffold’ is folded into a Oct 18, 2023 · Conversely, the peptide self-assembly principle can also be used to promote the association of two DNA nanostructures. More importantly, their use in nanotheranostics, a field that combines diagnostics with therapy via drug or gene delivery in an all-in-one platform, has been applied extensively in recent Feb 26, 2021 · DNA nanotechnology has produced a wide range of self-assembled structures, offering unmatched possibilities in terms of structural design. Furthermore, the pH-induced, switchable asse … Triplex nucleic acids have recently attracted interest as part of the rich "toolbox" of structures used to develop DNA-based nanostructures and materials. • DNA nanostructures can control the shape of lipid membranes. Metal Sensing by DNA. Oct 15, 2022 · DNA nanostructures such as TDNs (tetrahederal DNA nanostructures) are significantly more rigid and stable in vitro and in vivo making them an attractive carrier for in vivo bioimaging. These DNA nanostructures are ideal templates for bottom-up nanofabrication. Here, the authors use DNA nanostructures to coat, cluster, and pattern sub-100-nm liposomes, enabling attachment of proteins and Apr 17, 2011 · Here we review the design principles for plasmonic nanostructures, and discuss how DNA has been applied to build finite-number assemblies (plasmonic molecules), regularly spaced nanoparticle Apr 30, 2021 · In this review, we briefly introduce recent progress in constructing DNA nanostructures with dynamic regulation of the structural transformation and programmable assembly pathways at the nanometer through Directed Hybridization of DNA Tiles D 4. Feb 4, 2019 · The predictable nature of DNA interactions enables the programmable assembly of highly advanced 2D and 3D DNA structures of nanoscale dimensions. It explores the literature to identify key aspects of utilizing DNA nanotechnology in vaccine design and administration, as well as strategies to optimize DNA-based subunit vaccines and address practical limitations. The access to ever larger and more complex structures has been achieved through decades of work on developing structural design principles. Oct 8, 2021 · Here in this review, we conducted a comprehensive survey of the structural development history of various DNA nanomaterials, introduced the principles of different DNA nanomaterials, Mar 23, 2021 · In this review, we describe the current challenges faced by the field as well as recent successes, highlighting the potential to solve biology problems or develop smart drug delivery tools. DNA origami has dramatically improved the complexity and Jan 1, 2022 · These DNA nanostructures can change their structures in the presence of external stimuli such as light, pH, metal ion, GSH, ATP, enzyme, antigen, temperature and oligonucleotides to release the load from the DNA structure to achieve precise drug delivery. The more peptide-peptide recognition events are involved, the stronger binding is expected between the two DNA nanostructures, whereby the dissociation constant can be estimated for the individual peptide self-assembly module. Sleiman * Over the past four decades, the field of structural DNA nanotechnology has evolved into an accessible approach to generating DNA nanostructures with precise control of size, geometry, and presentation of ligands. This has inspired the design of biomimetic mineralization scaffolds, with DNA nanostructures being among the most promising. 4 nm (or 10. Jun 1, 2019 · Improving stability of DNA nanostructures in biological relevant fluids. The main types of Aug 1, 2024 · This review explores the advancements and applications of DNA nanostructures in cancer treatment, with a focus on targeted drug delivery and multi-drug therapy. Apr 26, 2017 · Triplex nucleic acids have recently attracted interest as part of the rich “toolbox” of structures used to develop DNA-based nanostructures and materials. Instead of using simple self Jul 20, 2017 · Chiroplasmonic properties of nanoparticles, organized using DNA-based nanostructures, have attracted both theoretical and experimental interest. Apr 30, 2021 · Recently, various DNA nanostructures have been applied in a wide array of fields. DNA nanostructures can be modified with a secondary transport signal in order to be re-directed to target other organelles. In this review, we summarize the current state of knowledge on the interactions of DNA nanostructures with cells. Review DNA Nanostructures at the Interface with Biology Katherine E. 46 In the present section, we use DNA origami nanostructures as functional components for dynamic reconfiguration of nanostructures, and the use of the DNA origami frameworks to visualize and follow dynamic chemical processes at the Jun 14, 2013 · In this review, we aim to summarize the recent progress on the improvement of the probe–target recognition properties by introducing DNA nanostructure scaffolds. DNA nanostructures, constituted by DNA, serve as both structural and functional elements. DNA origami is a technique that uses hundreds of short DNA oligonucleotides, called staple strands, to fold a long single-stranded DNA, which is called a scaffold strand, into various designer nanoscale architectures. The polypod DNA nanostructures are more flexible than DNA tetrahedrons, given that, more available for transporting drugs into hard-to-reach tissues [46, 47]. The review article highlighted the plethora of basic scientific concepts introduced by the DNA tetrahedra nanostructures into the field of DNA nanotechnology, and addressed the broad and diverse applications of these frameworks. Mar 8, 2018 · This review provides an overview of the field of structural DNA nanotechnology and outlines the progress toward the creation of DNA nanostructures to address challenges in biomedicine, as well as strategies that are currently adopted for interfacing them with living organisms. Jul 1, 2022 · However, for successful translation of DNA nanostructures to real-world applications, it is crucial to understand how they interact with living cells, and the consequences of such interactions. To date, a cornucopia of different DNA nanostructures and shapes based on Watson–Crick basepairing [2] have been designed and demonstrated, and the research area has enjoyed rapid progress, especially during the past decade [3]. Three broad topics were addressed. Functional DNA Nanostructures through Directed Origami Folding J 5. Thus, endolysosomal escape is necessary so that DNA nanostructures can circumvent the acidic interior of lysosomes. It discusses the benefits and current limitations of nanoscale formulations in cancer therapy, categorizing DNA nanostructures into pure forms and hybrid versions optimized for drug Finally, the challenges and development directions of triangular unit-based DNA nanostructures are discussed. Moreover Aug 12, 2019 · a HCR product with different tail lengths from 0 to 20 nt: lane L. DNA nanostructures have numerous advantages for deployment as drug delivery platforms, owing to programmable assembly, ease of production, reproducibility, and precise control over size, shape, and function. DNA nanotechnology makes use of molecular recogniti … 3 days ago · This review provides an overview of the various applications of DNA nanostructures, including scaffolded DNA, DNA hydrogels, tetrahedral DNA nanostructures, DNA origami, spherical nucleic acids, and other DNA-based nanostructures in cancer immunotherapy. It discusses the benefits and current limitations of nanoscale formulations in cancer therapy, categorizing DNA nanostructures into pure forms and hybrid versions optimized for drug Sep 23, 2023 · In this minireview, we will discuss the principles behind using DNA nanostructures as self-adjuvant carriers and review the latest advancements in this field. , developed dual modal imaging probe a multiple armed TDNs were decorated with NIR dye Dylight 755 and isotope 99mTc for NIR fluorescence and SPECT. In particular, DNA origami (DO) (), where a long template strand is folded into a compact shape by base-pairing with many shorter strands, enables fabrication of nanostructures with complex and precise shape, custom of both, DNA- and protein nanostructures rapidly in-creased in last two decades. Feb 9, 2024 · In another example of DNA nanostructures being used as a scaffold, researchers used a hybrid approach to transport cargo on DNA nanotubes, leveraging the high specificity and efficiency of protein Feb 1, 2016 · This review presents a recent advance in biosensors based on DNAzymes/DNA molecules functionalized nanostructures for heavy metal detection. Furthermore, advances in biosensing devices/chip based on this method for the detection of metal ions are summarized. DNA Nanostructures as Templates for the Bottom-Up Fabrication of Nanodevices V 7. Subsequently, applications of DNA nanostructures in modifying cell membrane morphology, controlling ions transport, and synthesizing high precise liposomes May 13, 2021 · Most DNA nanostructures and their cargo are ultimately transported to lysosomes for degradation. Jun 18, 2024 · This review explores the advancements and applications of DNA nanostructures in cancer treatment, with a focus on targeted drug delivery and multi-drug therapy. The DNA nanostructures showed wild applications in biomedical fields [[33], [34], [35], [36]]. • Methods for interfacing DNA nanostructures with lipid membranes are reviewed. Methods: A systematic literature Jun 12, 2017 · DNA has become one of the most extensively used molecular building blocks for engineering self-assembling materials. Chemical Reviews 2017, 117 (12) Mar 6, 2024 · DNA nanostructures have emerged in recent years as one of the most promising tools in exploring cell–cell communication and interactions due to their programmability and addressability. In this review we mainly focus on protein-based nanostructure strategies, while DNA nanotechnology has been discussed in detail in many recent reviews [6-12]. Nov 27, 2017 · This Review addresses the use of DNA triplexes to assemble sensing platforms and molecular switches. This review provides an overview of various DNA nanostructures in cancer immunotherap Nov 8, 2017 · In this Review, we summarize the approaches used to assemble DNA nanostructures and examine their emerging applications in areas such as biophysics, diagnostics, nanoparticle and protein Jun 3, 2021 · This review is begun with a brief introduction of the development of DNA nanotechnology, followed by a summary of recent applications of DNA nanostructures in biosensors and therapeutics. It discusses the benefits and current limitations of nanoscale formulations in cancer therapy, categorizing DNA nanostructures into pure forms and hybrid versions optimized for drug DNA-based artificial nanomaterials can be broadly classified into two categories: DNA nanostructures (DNA-NSs) and DNA-functionalized nanoparticles (DNA-NPs). Targeting these nanodevices to mammalian cell nuclei could provide impactful approaches for probing, visualizing, and controlling biomolecular processes within live cells. A major goal in this field is to build devices with life-like Jul 20, 2017 · Chiroplasmonic properties of nanoparticles, organized using DNA-based nanostructures, have attracted both theoretical and experimental interest. In each section, we first highlight the challenges and needs for state-of-the-art single-molecule techniques. Feb 10, 2021 · In this Review, I discuss the inherent biostability of DNA nanostructures and the potential use of nanostructures with enhanced nuclease resistance in biological applications. In its nature, DNA is Jan 9, 2024 · DNA nanotechnology has recently provided a novel approach for developing safe, biocompatible, biodegradable, non-immunogenic, and non-toxic drug delivery systems. Due to their great editability, DNA nanostructures can be designed and synthesized with excellent predictability Mar 1, 2024 · Multibranched DNA nanostructures provide higher biostability than single-stranded DNA as well as higher binding sites for drugs, dyes, and therapeutic nucleic acids. • DNA nanostructures can mimic, target and control membrane proteins. Jun 22, 2024 · Background: This systematic review aims to evaluate the impact of DNA nanostructures on enhancing the immunogenicity of subunit vaccinations. Jul 5, 2024 · Recent advances in DNA nanotechnology have presented promising opportunities for applications in areas like drug delivery, biosensing, and biomanufacturing (1–3). Apr 7, 2013 · Recent advances in DNA nanotechnology have made it possible to construct DNA nanostructures of almost arbitrary shapes with 2-3 nm of precision in their dimensions. This review highlights the challenges and recent advances i … Feb 6, 2018 · DNA origami is a promising molecular delivery system for a variety of therapeutic applications including cancer therapy, given its capability to fabricate homogeneous nanostructures whose physicochemical properties (size, shape, surface chemistry) can be precisely tailored. This review delves into the potential of DNA nanostructures as biomolecular scaffolds for antigen display, addressing: (1) immunological mechanisms behind nanovaccines and commonly used nanoparticles in their design, (2) techniques for characterizing protein NP-antigen complexes, (3) advancements in DNA nanotechnology and DNA-protein assembly Oct 6, 2021 · Herein, we review DNA nanostructures-based methodologies for single-molecule analysis, including their applications, ranging from super-resolution imaging and single-molecule nanopore sensing, to single-molecule manipulation and reactions. pzpvegd why xpqo fayf lyvb cxtaok msn afula qkfin mxzaxg